![]() ![]() Standardizing counts in the Neubauer chamberīackground information on cell counts Use of trypan blue Short protocol for an optimal count in a Neubauer chamber On this page, you find the following topics: A good overview of the subdivisions of the counting squiares in different chamber types is given here (with German labels) Optik Labor. The Neubauer improved and the Thoma chamber, however, are the ones most often used in cell culture. In general, many different kinds of chambers may be used to count cells. We also want to give hints where to start trouble shooting when problems with the counts arise. In the following paragraphs, we have outlined the most important points for counting with a hemocytometer. Counting of cells (eukaryotic and especially mammalian cells) in a hemocytometer is an old but still valuable method to quantify the cell number per mL in a suspension. ![]()
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